12/25/2022 0 Comments Canine minitube![]() ![]() The TRIS-based extender with EYP could not be improved by addition of lecithin and catalase however, in-vivo fertilization capacity of the here examined extenders remains to be investigated. We conclude that egg yolk is superior to EYP in the here investigated extenders. Addition of lecithin and catalase to EYP containing extenders decreased motility and increased complete apoptosis. ![]() Replacement of egg yolk by EYP increased the ROS and late apoptotic cells. ![]() Curvilinear velocity (VCL), DNA fragmentation, morphological abnormalities and zona binding ability did not differ between groups. Semen samples with TRIS-EY with a final concentration of 5% glycerol and 0.5% Equex STM paste showed best post thaw progressive motility (P), most intact cells, lowest percentage of ROS, acrosome damages, dead and apoptotic cells. After freezing and thawing, samples were analyzed by CASA and sperm chromatin structure assay (SCSA) reactive oxygen species (ROS), degree of apoptosis and zona binding ability were determined. After equilibration the second dilution step was done: samples with (I) were diluted with TRIS-EY with 7% glycerol and 1% Equex STM paste samples with (II) and (III) were divided in 2 aliquots each, and one part diluted with TRIS-EYP or TRIS-EYP-L, both containing 7% glycerol and 1% Equex STM paste, and the other one part with the same extenders containing additionally 300 I.U./mL catalase. Fractionated semen collection was done in 17 stud dogs and the sperm rich fraction diluted with different extenders in 2 steps: (I) TRIS-fructose-citric acid extender (TRIS) containing 20% egg yolk (EY) and 3% glycerol, (II) TRIS containing 20% egg yolk plasma (EYP) and 3% glycerol, and (III) TRIS containing 20% EYP and 0.8% lecithin (EYP-L) and 3% glycerol. We also tested whether the addition of lecithin and catalase to the EYP extenders would improve results. Hereby the semen is deposited directly in the uterus by means of an endoscopically guided flexible catheter.We compared the results of using egg yolk plasma (EYP) instead of egg yolk (EY) in a TRIS-based Equex STM Paste freezing extender system for dog semen. In general the results of inseminations with chilled semen can be just as good as a natural mating if the semen after collection is of good quality, the timing is good and the insemination is done by TCI. This is the minimal amount of semen needed for fertilisation (100-150 million sperms with good motility and morphology).įor optimal results the best way to organise matings with chilled semen is to ensure that there is a maximum timeframe between the collection and the insemination of 36 hours. The price of freezing is higher than chilling and the transportation of frozen semen is more costly because of the need of using special containers with liquid nitrogen (dry shippers).Īnother advantage of using chilled semen is the use of the total collection for shipping and insemination while with frozen semen a collection is most of the time divided into several breeding-units. The costs of using chilled semen in total (collection, extending, packaging, transportation) are less than using frozen semen. You can see a photo of the box shown here.Īfter arriving at the destination the semen can be stored in a refrigerator if more time is needed for the female to reach the optimum time period. For most destinations this is enough to send the semen without loss of quality. The USA and Canada are countries where we can send chilled canine semen to with good results.įor the actual transportation at 4 degrees centrigrade we use a disposable box that can hold the cooled semen for 2 days. In that way an objective documentation is warranted.Ĭhilled semen is very easy to use in case of a short period of stocking the semen or for distribution within Europe and to other countries where quarantine regulations make it impossible to do so. It is also possible to email the moving images and the morphology images. We can send this digitally to the owner of the male and the receiving party before sending the semen. Right after the collection we complete a quality assessment before and after extending. The extenders are: Canirep, Mofa and Minitube. We use different extenders depending on the time needed to cover and the specific semen factors. The capability of fertilising ova is most of the time much less possible. Depending on the quality of the semen, the extender and the cooling curve it is possible to keep the motility up to 10 days. You will find a unique blend of products for Arts & Crafts, Education. ![]() The preservation of semen for several days is possible by spinning it immediately after collection and adding a chilled semen extender and gradually cooling it to 4 degrees centrigrade. Buy Inner Liner for Minitube Bovine Artificial Vagina, Rough, 65cm, C36315 at Nasco. ![]()
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